Anti-IL-11 Antibody (A-9) recommended for detection of IL-11 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-IL-11 Antibody (A-9): sc-133063

Anti-IL-11 Antibody (A-9) is rated 4.0 out of 5 by 4.
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Datasheets

    • Anti-IL-11 Antibody (A-9) is a mouse monoclonal IgG2a κ IL-11 antibody, cited in 4 publications, provided at 200 µg/ml
    • raised against amino acids 31-199 of IL-11 of human origin
    • Anti-IL-11 Antibody (A-9) is recommended for detection of IL-11 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-IL-11 Antibody (A-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IL-11 (A-9): sc-133063.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for IL-11 Antibody (A-9) for WB applications. These reagents are now offered in bundles with IL-11 Antibody (A-9) (see ordering information below).
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Is this compatible with Flow Cytometry by indirect detection?

Asked by: ELEE
Thank you for your question. We do not recommend using the unconjugated antibody for flow cytometry because we have not tested the antibody yet in this application.
Answered by: Technical Support
Date published: 2017-11-15

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with IL-11 (A-9): sc-133063?

Asked by: cjMara
We recommend using one of our our exclusive Mouse IgG Binding Proteins conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-02-27
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Rated 3 out of 5 by from No stripe This might be blamed to the mishandling of WB that all the PVDF membranes were nothing on it. But the free sample was run out, It was difficult to verify the validity of this antibody.
Date published: 2018-06-14
Rated 4 out of 5 by from Nice band seen in WB using human recombinant IL Nice band seen in WB using human recombinant IL-11. -SCBT QC
Date published: 2015-05-30
Rated 4 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB. -SCBT QC
Date published: 2015-05-16
Rated 5 out of 5 by from ELISA publishable data with mouse MC3T3 ELISA publishable data with mouse MC3T3-E1 cells - SCBT Publication Review
Date published: 2014-09-28
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