IGFN1 Antibody (S-2)recommended for detection of IGFN1 of human origin by WB, IP, IF and ELISA

IGFN1 Antibody (S-2): sc-81855

IGFN1 Antibody (S-2) is rated 4.0 out of 5 by 2.
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Datasheets

    • IGFN1 Antibody (S-2) is a mouse monoclonal IgG2a (kappa light chain) provided at 100 µg/ml
    • raised against recombinant IGFN1 of human origin
    • recommended for detection of IGFN1 of human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IGFN1 (S-2): sc-81855.
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I am using IGFN1 (S-2): sc-81855 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Cweed
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Produced great immunofluorescence membrane Produced great immunofluorescence membrane and cytoplasmic staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-02-22
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