IGFBP3 Antibody (4)recommended for detection of IGFBP3 of human origin by WB, IP and IF

IGFBP3 Antibody (4): sc-135947

IGFBP3 Antibody (4) is rated 4.0 out of 5 by 4.
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Datasheets
    • IGFBP3 Antibody (4) is a mouse monoclonal IgG1 provided at 50 µg/0.5 ml
    • raised against amino acids 101-210 of IGFBP3 of human origin
    • recommended for detection of IGFBP3 of human origin by WB, IP and IF
    • See IGFBP3 (E-9): sc-374365 for IGFBP3 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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I am using IGFBP3 (4): sc-135947 for immunofluorescence, what dilution of this antibody should I use?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Produced clear immunofluorescence cytoplasmic Produced clear immunofluorescence cytoplasmic and extracellular staining in human fibroblast cells. -SCBT QC
Date published: 2015-04-23
Rated 4 out of 5 by from Good publishable data in WB in human serum Good publishable data in WB in human serum. -SCBT Publication Review
Date published: 2015-04-17
Rated 4 out of 5 by from Robust signal in WB in human plasma Robust signal in WB in human plasma. -SCBT QC
Date published: 2015-04-11
Rated 4 out of 5 by from Strong band in WB in human endothelial cell lines Strong band in WB in human endothelial cell lines. -SCBT Publication Review
Date published: 2015-01-28
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