IGFBP2 Antibody (C-10)recommended for detection of IGFBP2 of human origin by WB, IP, IF and ELISA

IGFBP2 Antibody (C-10): sc-25285

IGFBP2 Antibody (C-10) is rated 4.4 out of 5 by 7.
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Datasheets
    • IGFBP2 Antibody (C-10) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-75 of IGFBP2 of human origin
    • IGFBP2 Antibody (C-10) is recommended for detection of IGFBP2 of human origin by WB, IP, IF and ELISA
    • IGFBP2 Antibody (C-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IGFBP2 (C-10): sc-25285.
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What is the recommended starting dilution for Western blot for sc-25285: IGFBP2 (C-10) monoclonal antibody?

Asked by: Germaine
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 1 out of 5 by from Does not work in ELISA Two separate lots of this antibody failed to detection either endogenous (whole cell lysate) or recombinant hIGFBP2. The ELISA designation should be removed.
Date published: 2017-07-27
Rated 5 out of 5 by from Great monoclonal antibody Strong and specific band with very little background in various samples
Date published: 2017-02-07
Rated 5 out of 5 by from 效果蛮不错的 课题组的都觉得报出来效果可以,希望多做些活动,小样赠送什么的都可以呀的。。。。。。。。。。。。。。。
Date published: 2017-01-18
Rated 5 out of 5 by from Worked well in WB in mouse DF Worked well in WB in mouse DF-1 cells. -SCBT Publication Review
Date published: 2015-05-14
Rated 5 out of 5 by from Strong band in WB in T98G Strong band in WB in T98G, MES-SA/Dx5 and MIA PaCa-2 whole cell lysates. -SCBT QC
Date published: 2015-04-08
Rated 5 out of 5 by from Produced publishable data in WB with human Produced publishable data in WB with human microvascular endothelial cells -SCBT Publication Review
Date published: 2015-03-03
Rated 5 out of 5 by from WB published data with human GBM (U362 WB published data with human GBM (U362, YKG-1, Y98G, A172, KS-1, U87, YH-13, U373, and NYGM) -SCBT Publication Review
Date published: 2013-10-02
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