IGFBP1 Antibody (H-5)recommended for detection of IGFBP1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

IGFBP1 Antibody (H-5): sc-55474

IGFBP1 Antibody (H-5) is rated 4.9 out of 5 by 7.
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Datasheets
    • IGFBP1 Antibody (H-5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 121-240 of IGFBP1 of human origin
    • IGFBP1 Antibody (H-5) is recommended for detection of IGFBP1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • IGFBP1 Antibody (H-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IGFBP1 (H-5): sc-55474.
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Can IGFBP1 (H-5): sc-55474 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jerojero
Thank you for your question. Yes, IGFBP1 (H-5): sc-55474 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2018-04-02
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Rated 5 out of 5 by from Good antibody! This primary antibody was used to examine the IGFBP1 in human endometrial cancer cell line HEC-1-B by western blotting, and the specific band was observed.
Date published: 2017-05-08
Rated 4 out of 5 by from WB with none spicific band We could see one dim 30kd band but with other none specific band by WB
Date published: 2016-12-30
Rated 5 out of 5 by from Positive signal in WB in mouse transfected Positive signal in WB in mouse transfected 293T and HepG2 whole cell lysates. -SCBT QC
Date published: 2015-04-26
Rated 5 out of 5 by from Beautiful immunoperoxidase cytoplasmic staining Beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human placenta tissue. -SCBT QC
Date published: 2015-04-17
Rated 5 out of 5 by from Strong band in WB in HepG2 whole cell lysate Strong band in WB in HepG2 whole cell lysate. -SCBT QC
Date published: 2015-02-15
Rated 5 out of 5 by from Robust signal in WB in human recombinant IGFBP1 Robust signal in WB in human recombinant IGFBP1. -SCBT QC
Date published: 2015-02-03
Rated 5 out of 5 by from Good publishable data in WB in human HepG2 Good publishable data in WB in human HepG2 cells at 1:500 dilution. -SCBT Publication Review
Date published: 2015-01-09
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