IgE Antibody (MH25-1)recommended for detection of IgE of human origin by WB, IP, IF and IHC(P)

IgE Antibody (MH25-1): sc-52335

IgE Antibody (MH25-1) is rated 4.0 out of 5 by 1.
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Datasheets

    • IgE Antibody (MH25-1) is a mouse monoclonal IgG2b provided at 500 µl
    • raised against isolated human IgE
    • recommended for detection of IgE of human origin by WB, IP, IF and IHC(P)
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Can sc-52335: IgE (MH25-1) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Trav11
Thank you for your question. Yes, sc-52335: IgE (MH25-1) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24

How many ug of IgE are in 500ul of this product (sc-52335)?

Asked by: Euna
Thank you for your question. This product, sc-52335: IgE (MH25-1), is a mouse monoclonal with the isotype of IgG2b. The antibody is partially purified and the concentration of immunoglobulin has not been determined. The total volume provided is 500 µl. The antibody is recommended to detect human IgE.
Answered by: Technical Support
Date published: 2018-05-01
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Rated 4 out of 5 by from Positive detection of endogenous expression Positive detection of endogenous expression of IgE by WB using U266 whole cell lysate. -SCBT QC
Date published: 2013-11-02
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