IgE Antibody (4F4)recommended for detection of epsilon 3 domain of IgE of human origin by WB and IP

IgE Antibody (4F4): sc-51994

IgE Antibody (4F4) is rated 4.0 out of 5 by 1.
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Datasheets

    • IgE Antibody (4F4) is a mouse monoclonal IgG2a provided at 100 µg/ml
    • raised against IgE from myelomatous human serum
    • recommended for detection of epsilon 3 domain of IgE of human origin by WB and IP
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I am using IgE (4F4): sc-51994 antibody for immunoprecipitation, how much antibody should I use?

Asked by: AbPolly
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Strong signal Strong signal, doublet or triplet bands observed corresponding to IgE protein observed by WB. -SCBT QC
Date published: 2014-05-12
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