IgE Antibody (154/102) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
raised against IgE of human origin
IgE Antibody (154/102) is recommended for detection of IgE of human origin by WB and IP
IgE Antibody (154/102) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IgE (154/102): sc-53346.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Immunoglobulins are four-chain, Y-shaped, monomeric structures comprised of two identical heavy chains and two identical light chains held together through interchain disulfide bonds. The chains form two domains, the Fab (antigen binding) fragment and the Fc (constant) fragment. Immunoglobulin epsilon (IgE) exists as a monomer. The IgE heavy chain is an ε-chain, and the light chains are either κ- or λ-chains. IgE is significantly involved in the allergic response of the body. It binds to receptors on the surface of basophils, mast cells and activated eosinophils. One dominant functional activity of IgE is the sensitization of mast cells. IgE binds to the Fc ε RI receptor on the surface of mast cells, causing the cell to release chemicals that induce reactions such as sneezing and coughing. IgE also helps to protect the host against large parasites. It coats the surface of the parasite attracting eosinophils via the Fc ε RI receptor. The eosinophils can then attack the parasites that are too large to be ingested by phagocytes.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What is the recommended starting dilution for Western blot for sc-53346: IgE (154/102) monoclonal antibody?
Asked by: jerojero
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol:
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn
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