Ig kappa chain Antibody (KP-53)recommended for detection of Ig κ chain of human origin by WB, IP, IF and IHC(P)

Ig κ chain Antibody (KP-53): sc-59264

Ig κ chain Antibody (KP-53) is rated 5.0 out of 5 by 2.
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Datasheets

    • Ig κ chain Antibody (KP-53) is a mouse monoclonal IgG2b provided at 500 µl ascites
    • raised against purified Bence Jones kappa light chain protein of human origin
    • recommended for detection of Ig κ chain of human origin by WB, IP, IF and IHC(P); non cross-reactive with human lambdalight chains
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Can sc-59264: Ig κ chain (KP-53) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Trav11
Thank you for your question. Yes, sc-59264: Ig κ chain (KP-53) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Positive detection of endogenous expression Positive detection of endogenous expression of human Ig chain in PBL whole cell lysates by WB.
Date published: 2015-03-25
Rated 5 out of 5 by from Good specificity shown by using transfected Good specificity shown by using transfected and non-transfected 293T cells for over-expression of the human Ig chain protein via Western Blot analysis. -SCBT QC
Date published: 2013-01-19
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