IFT81 Antibody (A-3)recommended for detection of IFT81 of mouse, rat and human origin by WB, IP, IF and ELISA

IFT81 Antibody (A-3): sc-374272

IFT81 Antibody (A-3) is rated 2.3 out of 5 by 3.
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Datasheets

    • IFT81 Antibody (A-3) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 377-676 mapping at the C-terminus of IFT81 of human origin
    • IFT81 Antibody (A-3) is recommended for detection of IFT81 of mouse, rat and human origin by WB, IP, IF and ELISA
    • IFT81 Antibody (A-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IFT81 (A-3): sc-374272.
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What is the recommended starting dilution for Western blot for IFT81 (A-3): sc-374272 monoclonal antibody?

Asked by: Germaine
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-374272.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 1 out of 5 by from Did not work for IF in human RPE-hTERT cells This antibody did not work for IF (no cilia/centrosomal staning) in 4% PFA-fixed RPE-hTERT cells.
Date published: 2018-02-28
Rated 1 out of 5 by from N'a pas fonctionné en Immunofluorescence J'ai testé cet anticorp en Immunofluorescence, fixation MetOH et PFA, incubation 1h ou O/N à 4°C, je n'ai pas réussit à obtenir de signal sur des RPE-1 induit en ciliogenèse.
Date published: 2018-02-15
Rated 5 out of 5 by from Great Western blot data of IFT81 expression Great Western blot data of IFT81 expression in mouse testis and human testis tissue extracts. -SCBT QC
Date published: 2013-05-04
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