IFN-gamma Antibody (LLO6Z)recommended for detection of IFN-γ of human origin by IF, IHC(P), FCM and ELISA

IFN-γ Antibody (LLO6Z): sc-74108

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Datasheets

    • IFN-γ Antibody (LLO6Z) is a mouse monoclonal IgG2b provided at 100 µg/ml
    • raised against full length recombinant IFN-γ of human origin
    • recommended for detection of IFN-γ of human origin by IF, IHC(P), FCM and ELISA
    • See IFN-γ (E-10): sc-373727 for IFN-γ antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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请问这种抗体一般在什么肿瘤表达阳性率高?

Asked by: eraser
感谢您的提问。关于此问题的详细细节,欢迎通过:电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”,联系我们的技术支持部门。 Thank you for your inquiry. For more detailed information about this question, please contact our Asian Technical Service team at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 15
Date published: 2018-02-01

Can sc-74108: IFN-γ (LLO6Z) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Trav11
Thank you for your question. Yes, sc-74108: IFN-γ (LLO6Z) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24

please suggest the best secondary antibody for this product (IFN-γ Antibody (LLO6Z))

Asked by: nadiahistology
Thank you for your question. IFN-γ Antibody (LLO6Z) is a mouse monoclonal IgG2b and so we would recommend using either a conventional anti-mouse IgG secondary antibody, or preferably, one of our exclusive new mouse IgG binding proteins. This antibody has a kappa light chain, so you would want to choose the mouse IgG kappa binding protein (m-IgGκ BP) conjugated to appropriate molecule for your application. A link to the complete list of products is provided below. https://www.scbt.com/scbt/browse/Antibodies-Secondary-Antibodies/_/N-1hoaxk4
Answered by: Technical Support
Date published: 2017-01-20
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