IFN-gamma Antibody (F12)recommended for detection of both recombinant and native IFN-γ of human origin by WB, IP and IF

IFN-γ Antibody (F12): sc-52681

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Datasheets

    • IFN-γ Antibody (F12) is a mouse monoclonal IgG1 provided at 100 µg/ml
    • recommended for detection of both recombinant and native IFN-γ of human origin by WB, IP and IF; non cross-reactive with other cytokines or rodent interferons
    • See IFN-γ (E-10): sc-373727 for IFN-γ antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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I am using IFN-γ (F12): sc-52681 monoclonal antibody for immunofluorescence, what dilution of this antibody should I use?

Asked by: Randy McDonald
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-04-05
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