IFN-γ Antibody (E-10) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
specific for an epitope mapping between amino acids 25-50 at the N-terminus of IFN-γ of human origin
IFN-gamma Antibody (E-10) is recommended for detection of precursor and mature IFN-γ of human origin by WB, IP, IF and ELISA
IFN-gamma Antibody (E-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
blocking peptide, sc-373727 P
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IFN-γ (E-10): sc-373727.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Interferon (IFN)-γ is an antiviral and antiparasitic agent produced by CD4+/CD8+ lymphocytes and natural killer cells that undergo activation by antigens, mitogens or alloantigens. IFN-γ production modulates T cell growth and differentiation and inhibits the growth of B cells. Synthesis of IFN-γ is inducible by IL-2, FGF and EGF. The active form of IFN-γ is a homodimer with each subunit containing six helices. The dimeric structure of human IFN-γ is stabilized by non-covalent interactions through the interface of the helices. IFN-γ translated precursor is 166 amino acids, including the 23 amino acid secretory sequence. Multiple forms exist due to variable glycosylation and under non-denaturing conditions due to dimers and tetramers.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What application is the blocking peptide sc-373727 P appropriate for?
Asked by: Trav11
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here:
Rated 3 out of
better results with sc-8423pref. the sc-8423 product over this when working with mouse samples.
Date published: 2018-03-23
Rated 1 out of
Holy (and expensive) waterTried the free IFNgamma (E-10) in Western blot (1:100 as suggested by datasheet) with olfactory mesenchymal stem cells (oMSC) and mouse cortex protein extracts to check for signal in these two models. Not a single band was detected. Everything was done properly. I have tested 8 (eight!) mouse monoclonal antibodies from Santa Cruz and not a single one gave any band except one that gave an aspecific signal (I would like to upload a picture, but it is not allowing me). I will not loose more time with them and I strongly advice you to do the same. Good luck, fellow researchers.
Date published: 2017-11-17
Rated 3 out of
Good antibodyWorked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 4 out of
A GOOD PRODUCTI used the antibody in western blot, and to test the mice liver samples. So, that's good!
Date published: 2017-09-19
Rated 5 out of
very usefulIFN-gama antibody effect is good, immunohistochemical staining uniform, positive coloring significantly.
Date published: 2017-07-21
Rated 5 out of
Produced positive Western blot data of humanProduced positive Western blot data of human recombinant IFN- fusion protein. -SCBT QC
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn
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