IFN-gamma Antibody (4S.B3)recommended for detection of glycosylated and non-glycosyated IFN-γ of human origin by WB and IP

IFN-γ Antibody (4S.B3): sc-47700

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Datasheets

    • IFN-γ Antibody (4S.B3) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against interferon gamma derived from human leukocytes
    • recommended for detection of glycosylated and non-glycosyated IFN-γ of human origin by WB and IP
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See IFN-γ (E-10): sc-373727 for IFN-γ antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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How much sample do you recommend loading on the gel for Western blot detection using IFN-γ (4S.B3): sc-47700?

Asked by: cjMara
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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