IFN- alpha / beta R alpha Antibody (H-11) recommended for detection of IFN-α/βR α chain of mouse, rat and human origin by WB, IP and IF

IFN-α/βRα Antibody (H-11): sc-7391

IFN-α/βRα Antibody (H-11) is rated 4.6 out of 5 by 12.
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Datasheets

    • Anti-IFN-α/βRα Antibody (H-11) is a mouse monoclonal IgG1 (kappa light chain) IFN-α/βRα antibody provided at 200 µg/ml
    • raised against amino acids 458-557 mapping at the C-terminus (complete intracellular domain) of IFN-α/βRα of human origin
    • Anti-IFN- alpha / beta R alpha Antibody (H-11) is recommended for detection of IFN-α/βR α chain of mouse, rat and human origin by WB, IP and IF
    • Anti-IFN- alpha / beta R alpha Antibody (H-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IFN-α/βRα (H-11): sc-7391.

What is the total volume provided of IFN-α/βRα (H-11):sc-7391 monoclonal antibody?

Asked by: jenni
Thank you for your question. This antibody is provided as 200 µg in 1 ml total volume of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Support 4
Date published: 2017-01-13

What is the recommended fixation method for IF with IFN-α/βRα (H-11): sc-7391 monoclonal antibody?

Asked by: jenni
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support 4
Date published: 2017-01-13
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Rated 4 out of 5 by from Good antibody This anibody worked for WB with mouse tissue lysate.
Date published: 2020-02-05
Rated 4 out of 5 by from Good antibody This antibody worked very well with fixed frozen mouse tissue sections for IF
Date published: 2018-10-03
Rated 5 out of 5 by from Outstanding western and IF Very nice IF staining data; also strong western blot results
Date published: 2017-01-23
Rated 5 out of 5 by from Great staining results This antibody shows good staining results in IF for human.
Date published: 2017-01-14
Rated 4 out of 5 by from This has worked well in WB&IF&IP This has worked well in WB&IF&IP.
Date published: 2015-10-23
Rated 4 out of 5 by from This antibody works well and can be used This antibody works well and can be used for IP with human umbilical vein ECs. -SCBT Publication Review
Date published: 2015-06-10
Rated 5 out of 5 by from Good WB publishable data gathered with mouse HEK Good WB publishable data gathered with mouse HEK-293 cells. -SCBT Publication Review
Date published: 2015-04-11
Rated 5 out of 5 by from Publishable data in coimmunoprecipitation Publishable data in coimmunoprecipitation and Western blotting with this antibody -SCBT Publication Review
Date published: 2015-04-08
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