IFN-alpha Antibody (5D4)recommended for detection of all IFN-α subtypes of human origin by WB and IP

IFN-α Antibody (5D4): sc-80996

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Datasheets

    • IFN-α Antibody (5D4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against recombinant IFN-α of human origin
    • recommended for detection of all IFN-α subtypes of human origin by WB and IP
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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I am using IFN-α (5D4): sc-80996 antibody for immunoprecipitation, how much antibody should I use?

Asked by: cjMara
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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