IFIT3 Antibody (E-10)recommended for detection of IFIT3 of mouse, rat and human origin by WB, IP, IF and ELISA

IFIT3 Antibody (E-10): sc-393396

IFIT3 Antibody (E-10) is rated 5.0 out of 5 by 3.
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Datasheets

    • IFIT3 Antibody (E-10) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 286-490 mapping at the C-terminus of IFIT3 of human origin
    • IFIT3 Antibody (E-10) is recommended for detection of IFIT3 of mouse, rat and human origin by WB, IP, IF and ELISA
    • IFIT3 Antibody (E-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IFIT3 (E-10): sc-393396.
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What blocking solution should I use with IFIT3 (E-10): sc-393396 monoclonal antibody for Western blot?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from Sehr gute Ergebnisse im Westernblot. Antikörper funktioniert gut im Westernblot bei 1:1000 Verdünnung und Blocking in Rotiblock.
Date published: 2018-04-09
Rated 5 out of 5 by from Great antibody It detected endogenous IFIT3 in immortalized HFFs (1:500 dilution in 5% milk in TBS-0.1% Tween). Recommended!
Date published: 2017-10-28
Rated 5 out of 5 by from Very good Western blot data of IFIT3 expression Very good Western blot data of IFIT3 expression in HeLa, Hep G2 and Caki-1 whole cell lysates. -SCBT QC
Date published: 2013-04-26
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