IFI-16 Antibody (1G7)recommended for detection of IFI-16 of human origin by WB, IP, IF, IHC(P), FCM and ELISA

IFI-16 Antibody (1G7): sc-8023

IFI-16 Antibody (1G7) is rated 4.8 out of 5 by 17.
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Datasheets

    • IFI-16 Antibody (1G7) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-159 mapping at the N-terminus of IFI-16 of human origin
    • IFI-16 Antibody (1G7) is recommended for detection of IFI-16 of human origin by WB, IP, IF, IHC(P), FCM and ELISA
    • IFI-16 Antibody (1G7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IFI-16 (1G7): sc-8023.
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I work with mouse samples and need to do IHC. How can that work with this mouse monoclonal antibody?

Asked by: Cweed
We recommend using conjugated primary antibodies. This product, sc-8023, is available directly conjugated to FITC, AF488, and AF 647.
Answered by: TechService7
Date published: 2016-12-30
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Rated 5 out of 5 by from Very good I ordered the IFI16 Ab (1G7) about one month ago. I have done a couple of repeated experiments, it really works well in western blot. We treated U373 cells with reagent at different concentrations, and collected total proteins after 24 h treatment (unpublished data).
Date published: 2018-04-26
Rated 5 out of 5 by from Great Antibody for IFI16. Works for WB, IFA and IP Works for WB (1:1000), IFA (1:100) and IP (1:30). Human samples were used - B cell lymphoma (Cell lines), Endothelial cells (primary and cell lines).
Date published: 2017-11-07
Rated 5 out of 5 by from Good specificity We obtained a right band in the right molecular weight 1:1000 dilution for WB.
Date published: 2017-05-12
Rated 5 out of 5 by from Good antibody Strong band observed by WB using human tissue extract
Date published: 2017-04-19
Rated 5 out of 5 by from Excellent antibody! We have been using this antibody in many applications, IHC, IFL and ELISA, and always with excellent results. Highly recommended!
Date published: 2017-04-14
Rated 5 out of 5 by from works on macaque tissue Detects IFI-16 in paraffin embedded macaque retina tissue and by immunoblotting of retina lysates.
Date published: 2017-03-07
Rated 5 out of 5 by from Versitle Antibody IFI-16 Antibody (1G7) produced nice signal by WB, IHC(P), IF and FCM. This monoclonal is very versatile. Published in many citations! Highly recommended!
Date published: 2017-01-12
Rated 4 out of 5 by from Great for western blot Used 1/500 overnight for WB on cultured primary human fibroblast cell lysate. No non-specific bands detected and the triplet of bands around 110kDa (relative to Pageruler marker on a 10% gel) were all reduced with 2 independent siRNAs. Primary ab dilution stands re-use at least 5 times/1 month when stored at 4°C.
Date published: 2017-01-05
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