Hyaluronidase CAS: 37259-53-3

Hyaluronidase (CAS 37259-53-3)

Hyaluronidase | CAS 37259-53-3 is rated 4.7 out of 5 by 3.
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Alternate Names: Hyaluronidase is also known as Hyaluronate Lyase.
Application: Hyaluronidase is an enzyme that specifically cleaves hyaluronic acid, and can be used as media for the isolation and purifying of single cells.
CAS Number: 37259-53-3
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
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Hyaluronidase is an enzyme widely present in nature. In addition to humans, Hyaluronidase is found in the organs and bodily fluid of nematodes, leeches, snakes, scorpions, bees, spiders, wasps, hornets, caterpillars, lizards, and fish. Hyaluronidase is also present in the secretions of virus, bacteriophage, fungi and bacteria. In the preparation of DMEM/F-12 media, Hyaluronidase is used. Hyaluronidase is a component of digestion solution for the derivation of germline stem cells in certain types of cancer. Hyaluronidase cleaves hyaluronic acid at the β-D-GalNAc-(1→4)-β-D-GlcA bond, yielding 3-(4-deoxy-β-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine tetra- and hexasaccharides.


References

1. Ohya, T., and Kaneko, Y. Biochim. Biophys. Acta 198, 607, (1970) Abstract; Merck:Merck 13,4777;
2. A B Csoka et. al
Matrix biology : journal of the International Society for Matrix Biology, 20(8), undefined (2001-12-4)
3. Wen Min Lau et. al
Cancer research, 74(9), undefined (2014-3-13)
4. Tímea Váradi et. al
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 60(8), undefined (2012-5-9)

Formulation :
One ampule contains 300 units
Appearance :
Lyophilized powder
Physical State :
Solid
Derived From :
Streptomyces hyalurolyticus
Storage :
Store at -20° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
EC Number :
253-430-8

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Certificate of Analysis

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Hyaluronidase (CAS 37259-53-3)  Product Citations

See how others have used Hyaluronidase (CAS 37259-53-3). Click on the entry to view the PubMed entry .

Citations 1 to 3 of 3 total

PMID: # 24446084  Mima, K. et al. 2014. The British journal of surgery. 101: 269-76.

PMID: # 23391595  Lindwall, C. et al. 2013. Brain research. 1503: 62-77.

PMID: # 32442025  Am. J. Physiol. Heart Circ. Physiol.

Citations 1 to 3 of 3 total

How should the enzyme be reconstituted for long-term storage and at these conditions how long is the enzyme stable for?

Asked by: harding
Thank you for your question. The product has buffer present (it is lyophilized from 0.5 mM Tris buffer at pH 7), it is soluble in 1 mL of water and should be stored refrigerated; do not freeze since it is not known what effect freeze/thawing would have on the solutions. Unfortunately we do not have an estimate of stability time for refrigerated enzyme solutions.
Answered by: Tech Service
Date published: 2019-04-17
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Rated 5 out of 5 by from Excellent Product We bought this for our lab a few months ago and have been using it ever since. It comes in an easy to open ample and can be used for several treatments. It works great too!
Date published: 2020-10-13
Rated 4 out of 5 by from works as expected This hyaluronidase was cheaper and more readily available than options offered by some other companies, with same apparent enzyme activity. Documentation was not included, but technical support was very helpful in determining resuspension and storage methods.
Date published: 2017-11-06
Rated 5 out of 5 by from Yamaguchi et al Yamaguchi et al. (PubMed ID 26296532) used hyaluronidase to inhibit the activity of hyaluronic acid by cleaving. -SCBT Publication Review
Date published: 2015-04-01
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