Thank you for your question. This product, human liver extract: sc-363766, is provided ready to use as 500 µg protein in 200 µl SDS-PAGE Western blotting buffer. We recommend loading 50 µg (20 µl) per lane for use as a positive control in Western blotting. There is no need to dissolve the extract in any buffer. The sample vial should be boiled once prior to use.
Date published: 2018-03-29