human adipose tissue extract Western blotting positive control

human adipose tissue extract: sc-363750

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    • 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
    • Western blotting positive control
    • recommended use is 50 µg (20 µl) per lane
    • extracts should be stored at -20°C and repeated freezing and thawing should be minimized
    • sample vial should be boiled once prior to use
    • Not available in Germany.

Just to confirm, there is no need to add sample buffer or beta-mercaptoethanol to any of your human protein tissue extracts (e.g. human adipose tissue extract: sc-363750)? Just boil the 20 microliter aliquot and add to the gel? Thank you, John

Asked by: Anonymous
Thank you for your question. You are correct, these products come ready to use. There is no need to add any sample buffer. If you have any further questions, please contact Technical Service.
Answered by: Technical Service 3
Date published: 2020-11-13

Hi, how long do I boil prior to first use?

Asked by: Luis A
Thank you for your question. We recommend boiling at 95C for 3-5 minutes prior to first use.
Answered by: Technical Support
Date published: 2019-04-13
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