HSP 60 Antibody (SPM253)recommended for detection of HSP 60 of mouse, rat, human, bovine, porcine and canine origin by WB, IP, IF, IHC(P), FCM and ELISA

HSP 60 Antibody (SPM253): sc-65568

HSP 60 Antibody (SPM253) is rated 4.0 out of 5 by 1.
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Datasheets

    • HSP 60 Antibody (SPM253) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against recombinant HSP 60 of human origin, with epitope mapping to amino acids 383-447
    • recommended for detection of HSP 60 of mouse, rat, human, bovine, porcine and canine origin by WB, IP, IF, IHC(P), FCM and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See HSP 60 (LK1): sc-59567 for HSP 60 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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Can HSP 60 (SPM253): sc-65568 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Professor Griffin
Thank you for your question. Yes, HSP 60 (SPM253): sc-65568 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Antibody works well for WB in 293T samples Antibody works well for WB in 293T samples. -SCBT QC
Date published: 2015-03-30
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