HSP 60 Antibody (C-10)recommended for detection of HSP 60 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

HSP 60 Antibody (C-10): sc-376240

HSP 60 Antibody (C-10) is rated 5.0 out of 5 by 6.
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Datasheets

    • HSP 60 Antibody (C-10) is a mouse monoclonal IgM (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 533-569 near the C-terminus of HSP 60 of mouse origin
    • recommended for detection of HSP 60 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • blocking peptide, sc-376240 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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What application is the blocking peptide sc-376240 P appropriate for?

Asked by: Randy McDonald
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from an excelllent antibody for WB and IF This antibody works great for WB (1:1000 dilution, 40 ug U251 lysate) and IF (1:150 dilution formaldehyde-fixed HeLa cells).
Date published: 2017-07-05
Rated 5 out of 5 by from Clean blot This antibody really good staing of human rectum tissue, strong staining.
Date published: 2017-05-20
Rated 5 out of 5 by from Very good antibody Observed positive bands and strong signal in multiple cell lines and tissue samples with HSP 60 Antibody (C-10)
Date published: 2017-01-24
Rated 5 out of 5 by from IHC: Robust staining in paraffin IHC: Robust staining in paraffin-embedded human rectum. -SCBT QC
Date published: 2015-03-10
Rated 5 out of 5 by from Works well for IF applications Works well for IF applications, positive in HeLa cells. -SCBT QC
Date published: 2015-03-06
Rated 5 out of 5 by from Good specificity and signal strength with Good specificity and signal strength with little background using KNRK samples. -SCBT QC
Date published: 2015-01-07
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