HSP 47 Antibody (G-12)recommended for detection of HSP 47 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

HSP 47 Antibody (G-12): sc-5293

HSP 47 Antibody (G-12) is rated 4.9 out of 5 by 13.
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Datasheets

    • HSP 47 Antibody (G-12) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 129-300 of HSP 47 of human origin
    • HSP 47 Antibody (G-12) is recommended for detection of HSP 47 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • HSP 47 Antibody (G-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HSP 47 (G-12): sc-5293.
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Do you have the blocking peptide for HSP 47 Antibody (G-12): sc-5293?

Asked by: Manjunath Nayak
Thank you for the question. This antibody was raised against amino acids 129-300 of HSP 47 of human origin. A blocking peptide / recomb. protein fragment with a length of >170 aas would commonly be too long to efficiently block the antibody binding sites of this monoclonal antibody in a competition assay. Unfortunately, we therefore do not offer the corresponding blocking / negative control reagent. Instead, you might want to try a -NIC or CRISPR or siRNA negative control in case you are working with cultured cells.
Answered by: Technical Support Europe
Date published: 2018-01-31

I need to use an antibody to detect the protein in samples of canine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Thank you for your question Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Santa Cruz TS
Date published: 2017-01-27
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Rated 5 out of 5 by from Perfect antibody against Hsp47 Hsp47 is collagen-specific ER chaperone. We used this antibodies for multiple applications including western blotting, immunocytostaining and immunofluorescence of tissues (paraffin). All staining was specific and strong. Specificity of this antibody was validated using inhibitor of up-stream molecule (site-1 protease). I strongly suggest to purchase this clone from Santa Cruz.
Date published: 2019-08-28
Rated 5 out of 5 by from excellent ウエスタンブロットおよび蛍光免疫染色に使用。ノンスペバンドもなく、また低濃度でも十分に検出できます。
Date published: 2019-04-24
Rated 5 out of 5 by from Antibodies nicely working for WB Band of appropriate size after immunobloting of LN229 cell lystes.
Date published: 2018-02-16
Rated 5 out of 5 by from Good antibody This antibody shows great reactivity. It is highly specific
Date published: 2017-09-19
Rated 5 out of 5 by from Western Blot We used the HSP 47 antibody for Western Blot, the stripes are very fine at 47kda.
Date published: 2017-05-31
Rated 5 out of 5 by from strong recommend antibody We observed clear band when diluted 1:500 ,Great help to us, strongly recommended.
Date published: 2017-05-28
Rated 5 out of 5 by from works very good We use it usually , works very well in WB at 1:500 dilution.
Date published: 2017-02-26
Rated 5 out of 5 by from A strong HSP 47 monoclonal choice! This excellent antibody has great reactivity in a wide range of applications. It demonstrates strong bands in a Western blot and has provide nice immunofluoresence and Immunoperoxidase staining . This product is well characterized in the primary literature.
Date published: 2016-12-20
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