Anti-HoxD3 Antibody (4AY) recommended for detection of HoxD3 of mouse, rat and human origin by WB and IP

Anti-HoxD3 Antibody (4AY): sc-130378

Anti-HoxD3 Antibody (4AY) is rated 4.0 out of 5 by 1.
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Datasheets

    • Anti-HoxD3 Antibody (4AY) is a mouse monoclonal IgG2a (kappa light chain) HoxD3 antibody provided at 100 µg/ml
    • raised against recombinant HoxD3 of human origin
    • recommended for detection of HoxD3 of mouse, rat and human origin by WB and IP
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HoxD3 (4AY): sc-130378.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for HoxD3 Antibody (4AY). This work is in progress.
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Using HOXD3 (4AY) antibody for IF. How much antibody would you recommend?

Asked by: StuTech
Thank you for your question. We have not yet tested this antibody for immunofluorescent staining and so IF is not a recommended application at this time. If you wish to test the antibody for IF, we would typically recommend a starting dilution of 1:50 (range of 1:25-1:500) for IF.
Answered by: Technical Support
Date published: 2019-02-27

I am using HoxD3 (4AY): sc-130378 antibody for immunoprecipitation, how much antibody should I use?

Asked by: Cweed
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Strong doublet observed in western blot analysis Strong doublet observed in western blot analysis of HoxD3 expression in HeLa nuclear extract. -SCBT QC
Date published: 2013-09-07
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