Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HoxC6 Double Nickase Plasmid (h): sc-403393-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HoxC6 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HoxC6 Double Nickase Plasmid (h) and HoxC6 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HOXC6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HoxC6 Antibody (B-7): sc-376330
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HoxC6 Double Nickase Plasmid (h) | sc-403393-NIC | 20 µg | $410.00 | |||
HoxC6 Double Nickase Plasmid (h2) | sc-403393-NIC-2 | 20 µg | $410.00 |
HOXC6 encodes the homeobox transcription factor HoxC6, a sequence-specific DNA-binding regulator that coordinates anterior–posterior patterning and lineage specification during development. In human cells, HoxC6 modulates transcriptional programs linked to cell fate decisions, differentiation, and tissue morphogenesis by integrating with broader HOX regulatory networks and chromatin-mediated control of gene expression. Dysregulated HOXC6 expression has been associated with altered differentiation states and transcriptional reprogramming observed in multiple tumor types, supporting its relevance to studies of oncogenic signaling and developmental gene network misregulation. As a nuclear transcription factor, HoxC6 is frequently investigated in contexts such as epithelial–mesenchymal dynamics, proliferation control, and stem-like phenotypes through downstream target gene regulation.
HoxC6 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HOXC6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HOXC6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HOXC6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HOXC6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.