Anti-HoxC4 Antibody (G-12) recommended for detection of HoxC4 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-HoxC4 Antibody (G-12): sc-398460

Anti-HoxC4 Antibody (G-12) is rated 4.0 out of 5 by 3.
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Datasheets

    • Anti-HoxC4 Antibody (G-12) is a mouse monoclonal IgG1 (kappa light chain) HoxC4 antibody provided at 200 µg/ml
    • raised against amino acids 59-134 mapping within an internal region of HoxC4 of human origin
    • Anti-HoxC4 Antibody (G-12) is recommended for detection of HoxC4 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-HoxC4 Antibody (G-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HoxC4 (G-12): sc-398460.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for HoxC4 Antibody (G-12) for WB applications. These reagents are now offered in bundles with HoxC4 Antibody (G-12) (see ordering information below).
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For Western Blot, is it recommended to use denatured or non-denatured conditions with HoxC4 (G-12): sc-398460 monoclonal antibody?

Asked by: Trav11
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-02-27
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Rated 3 out of 5 by from Works OK, though I cannot call it a clean band Used at a dilution of 1:500 for neonatal mouse lung samples. The quality can be improved if I increase the concentration
Date published: 2019-08-27
Rated 4 out of 5 by from Works for western blotting in mouse lungs Used at a dilution of 1:500 in neonatal mouse lungs, and incubated overnight at 4oC
Date published: 2019-07-16
Rated 5 out of 5 by from Good Western blot data of HoxC4 expression in non Good Western blot data of HoxC4 expression in non-transfected and human HoxC4 transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-10-23
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