HoxA9 Double Nickase Plasmid (h): sc-401583-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HoxA9 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HoxA9 Double Nickase Plasmid (h) and HoxA9 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HOXA9. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HoxA9 Antibody (HOX5I043): sc-81291
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HoxA9 Double Nickase Plasmid (h) | sc-401583-NIC | 20 µg | $410.00 | |||
HoxA9 Double Nickase Plasmid (h2) | sc-401583-NIC-2 | 20 µg | $410.00 |
HOXA9 encodes the homeobox transcription factor HoxA9, a key regulator of anterior–posterior patterning and hematopoietic lineage commitment through sequence-specific DNA binding and transcriptional control of developmental gene programs. In human cells, HoxA9 helps coordinate proliferation and differentiation by modulating networks that intersect with stem cell maintenance, myeloid maturation, and chromatin-dependent transcriptional regulation. Dysregulated HOXA9 expression is frequently associated with leukemogenic transcriptional states and altered self-renewal, making it a widely studied node in hematopoietic malignancy models. Its activity is also examined in developmental biology and epigenetic studies to understand context-dependent enhancer usage and gene regulatory circuitry.
HoxA9 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HOXA9 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HOXA9. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HOXA9 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HOXA9-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.