Anti-HoxA5 Antibody (C-11) recommended for detection of HoxA5 of human origin by WB, IP, IF and ELISA

Anti-HoxA5 Antibody (C-11): sc-365784

Anti-HoxA5 Antibody (C-11) is rated 5.0 out of 5 by 1.
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Datasheets

    • Anti-HoxA5 Antibody (C-11) is a mouse monoclonal IgG1 κ HoxA5 antibody, cited in 5 publications, provided at 200 µg/ml
    • raised against amino acids 56-180 mapping within an internal region of HoxA5 of human origin
    • HoxA5 Antibody (C-11) is recommended for detection of HoxA5 of human origin by WB, IP, IF and ELISA
    • Anti-HoxA5 Antibody (C-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-365784 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HoxA5 (C-11): sc-365784.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for HoxA5 Antibody (C-11) for WB applications. These reagents are now offered in bundles with HoxA5 Antibody (C-11) (see ordering information below).

What is the recommended starting dilution for Western blot for sc-365784: HoxA5 (C-11) monoclonal antibody?

Asked by: jerojero
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Good Western blot data of HoxA5 expression in non Good Western blot data of HoxA5 expression in non-transfected and human HoxA5 transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-04-03
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