Anti-Hox11 Antibody (1D7) recommended for detection of Hox11 of mouse, rat and human origin by WB and IP

Anti-Hox11 Antibody (1D7): sc-12760

Anti-Hox11 Antibody (1D7) is rated 3.5 out of 5 by 4.
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Datasheets

    • Anti-Hox11 Antibody (1D7) is a mouse monoclonal IgG1 Hox11 antibody, cited in 4 publications, provided at 200 µg/ml
    • epitope mapping within amino acids 261-330 of Hox11 of human origin
    • Anti-Hox11 Antibody (1D7) is recommended for detection of Hox11 of mouse, rat and human origin by WB and IP
    • Anti-Hox11 Antibody (1D7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-12760 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Hox11 (1D7): sc-12760.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Hox11 Antibody (1D7). This work is in progress.
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When should the TransCruz reagent sc-12760 X be used instead of the standard form sc-12760?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from Published ICC data from murine T cells Published ICC data from murine T cells. -SCBT Publication Review
Date published: 2015-02-24
Rated 3 out of 5 by from Positive results in western blot analysis Positive results in western blot analysis of Hox11 expression in ALL-SIL whole cell lysate and Hox11 expression in ALL-SIL whole cell lysate immunoprecipitated with Hox11 (1D7): sc-12760 and detected by Western blot with the same antibody. -SCBT QC
Date published: 2015-02-18
Rated 4 out of 5 by from Worked well to immunoprecipitate and Hox11 Worked well to immunoprecipitate and Hox11 from murine lymphomas/also used for western blot verification. -SCBT Publication ReviewIP/WB
Date published: 2015-01-31
Rated 4 out of 5 by from Used for IP/WB with HOX11 Used for IP/WB with HOX11-3T3 fibroblasts, Jurkat, and ALL-SIL cells. -SCBT Publication Review
Date published: 2013-02-15
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