Hoechst 33342, UltraPure gradeA lipophilic fluorescent stain for DNA labeling

Hoechst 33342, UltraPure grade (CAS 23491-52-3)

Hoechst 33342, UltraPure grade | CAS 23491-52-3 is rated 5.0 out of 5 by 2.
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Synonym: Bisbenzimide
Application: A lipophilic fluorescent stain for DNA labeling
CAS Number: 23491-52-3
Molecular Weight: 561.93
Molecular Formula: C27H28N6O•3HCl
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Hoechst 33342 is a fluorescent nucleic acid stain useful for DNA labeling in fluorescence microscopy and flow cytometry. The compound is excited by ultraviolet light at around 350 nm, and emits blue/cyan fluorescence light around an emission maximum at 461 nm. Hoechst 33342 is more cell permeable than Hoechst 33258 (sc-391053) due to an extra ethyl group in the structure increasing lipophilicity. Hoechst dyes may be used on live or fixed cells, and are often used as a substitute for another nucleic acid stain, DAPI (sc-3598). Also commonly used to visualize nuclei and mitochondria.

Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in water and DMSO.
Storage :
Desiccate at 4° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
PubChem CID :
1464
SMILES :
CCOC1=CC=C(C=C1)C2=NC3=C(N2)C=C(C=C3)C4=NC5=C(N4)C=C(C=C5)N6CCN(CC6)C

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Hoechst 33342, UltraPure grade  Product Citations

See how others have used Hoechst 33342, UltraPure grade. Click on the entry to view the PubMed entry .

Citations 1 to 7 of 7 total

PMID: # 27096074  Li, L. et al. 2016. Cell & bioscience. 6: 26.

PMID: # 27190250  Wei, C. et al. 2016. Exp. Biol. Med. (Maywood). 241: 1505-15.

PMID: # 26453790  Zhang, Y. et al. 2015. International journal of pharmaceutics. 496: 392-400.

PMID: # 24368570  Nguyen, VT. et al. 2014. Marine drugs. 12: 69-87.

PMID: # 23546871  Song, K. et al. 2013. Applied biochemistry and biotechnology. 170: 459-70.

PMID: # 22846576  Luo, Y. et al. 2012. Biochem. Biophys. Res. Commun. 425: 473-7.

PMID: # 19107327  Zhu, Y. et al. 2009. Molecular and cellular biochemistry. 324: 117-29.

Citations 1 to 7 of 7 total
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Rated 5 out of 5 by from Worked very well in our lab Worked very well in our lab - used for DNA stain.
Date published: 2015-09-04
Rated 5 out of 5 by from A cell permeable dye used for DNA labeling A cell permeable dye used for DNA labeling. This Hoechst dye can be used on live and fixed cells alike. Functionality of this dye is demonstrating by counterstaining human retinal pigment epithelial cells (blue fluorescence). -SCBT QC
Date published: 2015-05-15
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