Hoechst 33258, UltraPure grade CAS: 23491-45-4
MF: C25H24N6O•3HCl
MW: 533.88
A fluorescent stain useful for DNA labeling.

Hoechst 33258, UltraPure grade (CAS 23491-45-4)

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Alternate Names: Bisbenzimidazole trihydrochloride
Application: Hoechst 33258, UltraPure grade is a fluorescent stain useful for DNA labeling
CAS Number: 23491-45-4
Molecular Weight: 533.88
Molecular Formula: C25H24N6O•3HCl
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
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Hoechst 33258 is a fluorescent nucleic acid stain useful for DNA labeling in fluorescence microscopy and flow cytometry. The compound is excited by ultraviolet light at around 350 nm, and emits blue/cyan fluorescence light around an emission maximum at 461 nm. Hoechst 33358 is less cell permeable than Hoechst 33342 (sc-391054), however this dye also allows for quantitative measurements when plotted in a standard emission-to-content curve. Hoechst dyes may be used on live or fixed cells, and are often used as a substitute for another nucleic acid stain, DAPI (sc-3598). Also commonly used to visualize nuclei and mitochondria.

Appearance :
Powder
Physical State :
Solid
Storage :
Desiccate at -20° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
PubChem CID :
SMILES :
CN1CCN(CC1)C2=CC3=C(C=C2)N=C(N3)C4=CC5=C(C=C4)NC(=C6C=CC(=O)C=C6)N5.Cl.Cl.Cl

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Certificate of Analysis

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Hoechst 33258, UltraPure grade (CAS 23491-45-4)  Product Citations

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PMID: # 31173390  2019. J. Bone Miner. Res.

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