Anti-hnRNP F/H Antibody (1G11) recommended for detection of hnRNP F and hnRNP H of mouse, rat, human and Xenopus origin by WB, IP, IF and IHC(P)

Anti-hnRNP F/H Antibody (1G11): sc-32310

Anti-hnRNP F/H Antibody (1G11) is rated 4.9 out of 5 by 8.
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Datasheets

    • Anti-hnRNP F/H Antibody (1G11) is a mouse monoclonal IgG1 λ hnRNP F/H antibody, cited in 15 publications, provided at 200 µg/ml
    • raised against purified recombinant hnRNP F/H of human origin
    • hnRNP F/H Antibody (1G11) is recommended for detection of hnRNP F and hnRNP H of mouse, rat, human and Xenopus origin by WB, IP, IF and IHC(P)
    • Anti-hnRNP F/H Antibody (1G11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of hnRNP F/H (1G11): sc-32310.
    • m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for hnRNP F/H Antibody (1G11) for WB and IHC(P) applications. This reagent is now offered in a bundle with hnRNP F/H Antibody (1G11) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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hello. i purified recombinant hnRNP-H, and reacted it with your antibody. but i always see 3 bends (~55kDa, ~40kDa, ~25kDa). i wanted to ask what is the epitope of the antibody, it may help me to understand the results. thank you Naomi

Asked by: Naomita613
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2020-03-03

I am using hnRNP F/H (1G11): sc-32310 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Randy McDonald
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from WBに使用 WBにて使用した結果です. 条件は写真に記しています。 HEK293T細胞10cm 1枚分のタンパク量です。
Date published: 2020-11-04
Rated 5 out of 5 by from Excellent Antibody! I received the free sample, and used for WB on DU145 lysate. I obtained a very satisfatory result, the bands were very strong, specific ! Love it so much :) Highly recommend to you!
Date published: 2018-02-02
Rated 4 out of 5 by from Good for WB it works well for WB in our lab, not a bad choice.
Date published: 2017-09-18
Rated 5 out of 5 by from Wonderful detection by WB This antibody works in WB using human MSC lysate from different conditions.It detects both F/H isoforms clearly.
Date published: 2017-05-17
Rated 5 out of 5 by from Published data for WB in human epidermoid Published data for WB in human epidermoid carcinoma A431 cells. -SCBT Publication Review
Date published: 2015-05-18
Rated 5 out of 5 by from Robust signal in WB in Jurkat and KNRK nuclear Robust signal in WB in Jurkat and KNRK nuclear extracts. -SCBT QC
Date published: 2015-04-12
Rated 5 out of 5 by from Published data for WB in rat PC12 cells Published data for WB in rat PC12 cells. -SCBT Publication Review
Date published: 2015-02-10
Rated 5 out of 5 by from Produced great immunoperoxidase nuclear staining Produced great immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human duodenum tissue. -SCBT QC
Date published: 2015-01-28
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