Anti-hnRNP F Antibody (3H4) recommended for detection of hnRNP F of human origin by WB, IP, IF and ELISA

Anti-hnRNP F Antibody (3H4): sc-32309

Anti-hnRNP F Antibody (3H4) is rated 4.3 out of 5 by 4.
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Datasheets

    • Anti-hnRNP F Antibody (3H4) is a mouse monoclonal IgG1 κ hnRNP F antibody, cited in 3 publications, provided at 200 µg/ml
    • raised against full length recombinant hnRNPF of human origin
    • hnRNP F Antibody (3H4) is recommended for detection of hnRNP F of human origin by WB, IP, IF and ELISA
    • Anti-hnRNP F Antibody (3H4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of hnRNP F (3H4): sc-32309.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for hnRNP F Antibody (3H4) for WB applications. These reagents are now offered in bundles with hnRNP F Antibody (3H4) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with hnRNP F (3H4): sc-32309 monoclonal antibody?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Nice nuclear IF staining of HeLa cells We used this antibody for immunoflourescence staining of hnRNP-F. We were able to get nice signal intensity and images.
Date published: 2017-02-07
Rated 4 out of 5 by from Good specificity for WB in human hnRNP F Good specificity for WB in human hnRNP F transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-04-09
Rated 4 out of 5 by from Produced great immunofluorescence nuclear Produced great immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-15
Rated 4 out of 5 by from Good band in WB in K Good band in WB in K-562 nuclear extract. -SCBT QC
Date published: 2015-02-07
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