Anti-hnRNP E2 Antibody (23-G) recommended for detection of hnRNP E2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-hnRNP E2 Antibody (23-G): sc-101136

Anti-hnRNP E2 Antibody (23-G) is rated 4.6 out of 5 by 10.
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Datasheets

    • hnRNP E2 Antibody (23-G) is a mouse monoclonal IgG2a κ, cited in 8 publications, provided at 100 µg/ml
    • raised against recombinant hnRNP E2 of human origin
    • recommended for detection of hnRNP E2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of hnRNP E2 (23-G): sc-101136.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for hnRNP E2 Antibody (23-G) for WB and IHC(P) applications. These reagents are now offered in bundles with hnRNP E2 Antibody (23-G) (see ordering information below).
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why does your PCBP2 western blot result have 2 bands? is the bottom band non-specific band?

Asked by: hrchang
Thank you for your question.The bands are most likely due to some post-translational modifications. pH of the gel buffers and/or running buffers can also sometimes cause slight doublets on Western blots.
Answered by: Tech Service
Date published: 2020-01-14

Can sc-101136: hnRNP E2 (23-G) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: cjMara
Thank you for your question. Yes, sc-101136: hnRNP E2 (23-G) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from WBにて使用しました。 WBにて使用した結果です. 条件は写真に記しています。 HEK293T細胞10cm 1枚分のタンパク量です。
Date published: 2020-11-04
Rated 5 out of 5 by from hnRNA E2 cellular location nucleolar location of hnRNPE2, very good to stain.
Date published: 2018-02-19
Rated 5 out of 5 by from good band It gives strong signal bands in mouse brain, an intense band of expected size can be seperated,1:500 diluted.
Date published: 2017-08-26
Rated 5 out of 5 by from western blot analysis Detection of hnRNP E2 expression in K562 cells by hnRNP this antibody,we got specific band at 40kda,I suggest use this sample as a positive control.
Date published: 2017-07-15
Rated 5 out of 5 by from Well characterized hnRNP ab for WB, IF & IHC This is a solid choice for the detection of hnRNP when using WB, IF or IHC(P) There are several citations in the primary literature supporting its use as well as strong WB band in K-562 whole cell lysate.
Date published: 2017-01-18
Rated 4 out of 5 by from Used in WB in human samples Used in WB in human samples, worked fine.
Date published: 2015-07-17
Rated 4 out of 5 by from Produced robust immunoperoxidase nuclear Produced robust immunoperoxidase nuclear and cytoplasmic staining in formalin-fixed, paraffin-embedded human testis tissue. -SCBT QC
Date published: 2015-03-26
Rated 5 out of 5 by from Great monoclonal antibody Great monoclonal antibody, works perfect for K-562 whole cell lysate. -SCBT QC
Date published: 2015-02-21
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