hnRNP E1 Antibody (F-6) recommended for detection of hnRNP E1 of human origin, and hnRNP E2 of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA

hnRNP E1 Antibody (F-6): sc-393076

hnRNP E1 Antibody (F-6) is rated 5.0 out of 5 by 4.
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Datasheets

    • Anti-hnRNP E1 Antibody (F-6) is a mouse monoclonal IgG1 (kappa light chain) hnRNP E1 antibody provided at 200 µg/ml
    • raised against amino acids 171-280 mapping within an internal region of hnRNP E1 of human origin
    • hnRNP E1 Antibody (F-6) is recommended for detection of hnRNP E1 of human origin, and hnRNP E2 of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
    • Anti-hnRNP E1 Antibody (F-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of hnRNP E1 (F-6): sc-393076.
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Can hnRNP E1/E2 (F-6): sc-393076 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Cweed
Thank you for your question. Yes, hnRNP E1/E2 (F-6): sc-393076 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Worked well for Western with mouse hnRNP Worked well for Western with mouse hnRNP E1 transfected 293T whole cell lysates and Sol8 nuclear extract. -SCBT QC
Date published: 2015-05-04
Rated 5 out of 5 by from IHC: Positive staining using paraffin IHC: Positive staining using paraffin-embedded human appendix tissue. -SCBT QC
Date published: 2015-04-20
Rated 5 out of 5 by from Single strong bands detected in K Single strong bands detected in K-562 and HeLa whole cell lysates. -SCBT QC
Date published: 2015-03-29
Rated 5 out of 5 by from Produced superb immunoperoxidase nuclear Produced superb immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human nasopharynx tissue. -SCBT QC
Date published: 2015-01-15
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