Anti-hnRNP C1/C2 Antibody (4F4) recommended for detection of hnRNP C1 and hnRNP C2 of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-hnRNP C1/C2 Antibody (4F4): sc-32308

Anti-hnRNP C1/C2 Antibody (4F4) is rated 4.4 out of 5 by 13.
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Datasheets

    • Anti-hnRNP C1/C2 Antibody (4F4) is a mouse monoclonal IgG1 κ hnRNP C1/C2 antibody, cited in 58 publications, provided at 200 µg/ml
    • raised against RNPs eluted from oligo (dT) cellulose column
    • Anti-hnRNP C1/C2 Antibody (4F4) is recommended for detection of hnRNP C1 and hnRNP C2 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • Anti-hnRNP C1/C2 Antibody (4F4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of hnRNP C1/C2 (4F4): sc-32308.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for hnRNP C1/C2 Antibody (4F4) for WB and IHC(P) applications. These reagents are now offered in bundles with hnRNP C1/C2 Antibody (4F4) (see ordering information below).
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Can I use hnRNP C1/C2: sc-32308 for RIP assay of cultured rat cells as in PMID: # 28264987 ?

Asked by: kiwai
Thank you for your question. We have not tested this antibody for RIP assay. However, this publication does indicate that this specific antibody was successfully used for IP, RIP and Western blot.
Answered by: Technical Support
Date published: 2018-12-17

Can I use hnRNP C1/C2 (4F4): sc-32308 mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: jenni
Yes. We recommend using a directly conjugated monoclonal antibody. AQP1 (B-11): sc-25287 monoclonal antibody is available directly conjugated to either HRP (sc-32308 HRP), phycoerythrin (sc-32308 PE), fluorescein (sc-32308 FITC), Alexa Fluor® 488 (sc-32308 AF488) or Alexa Fluor® 647 (sc-32308 AF647).
Answered by: Technical Support 12
Date published: 2017-01-09
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Rated 5 out of 5 by from excellent in WB WB in cell lysate of MDA dilution 1:1000 Clear and well detection of a band at the correct molecular weight
Date published: 2019-04-24
Rated 2 out of 5 by from can not be detected can not be detected by wetern blot.quite disappointing.
Date published: 2017-10-10
Rated 5 out of 5 by from user comment I used this antibody to do immunofluorescence at 1:500 the previous month,colon cancer cells.Fluorescence intensity is very strong.This antibody is great.
Date published: 2017-08-14
Rated 5 out of 5 by from 非常好用的抗体 该抗体功能较多,很实用,做过IP,很好拉相互作用蛋白,200ul体积的包装基本整个实验都够了。表达很好,特异性较好,使用时wb抗体浓度可以1:1000就能有较好的条带
Date published: 2017-05-09
Rated 5 out of 5 by from Great for western blot and IP Used at 1:400 for Western blot and yielded nice, specific staining. Successfully IPed the endogenous protein at 0.4ug antibody per 200ug lysate.
Date published: 2017-02-11
Rated 5 out of 5 by from Strong WB and IHC Great antibody; strong western blot and IHC staining data
Date published: 2017-01-11
Rated 5 out of 5 by from Very Strong Signal in WB This antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC. Strong, clear signal with little to no background.
Date published: 2017-01-04
Rated 4 out of 5 by from Produced wonderful immunoperoxidase nuclear Produced wonderful immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-03-10
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