Anti-HMGI-C Antibody (2421C6a) recommended for detection of HMGI-C of mouse, rat and human origin by WB and IP

Anti-HMGI-C Antibody (2421C6a): sc-130024

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Anti-HMGI-C Antibody (2421C6a) is rated 3.67 out of 5 by 3.
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Datasheets

    • Anti-HMGI-C Antibody (2421C6a) is a mouse monoclonal IgG1 κ HMGI-C antibody, cited in 7 publications, provided at 100 µg/ml
    • raised against a recombinant protein corresponding to the C-terminus of HMGI-C of human origin
    • recommended for detection of HMGI-C of mouse, rat and human origin by WB and IP
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HMGI-C (2421C6a): sc-130024.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for HMGI-C Antibody (2421C6a) for WB applications. These reagents are now offered in bundles with HMGI-C Antibody (2421C6a) (see ordering information below).
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I am using HMGI-C (2421C6a): sc-130024 antibody for immunoprecipitation, how much antibody should I use?

Asked by: Trav11
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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