Anti-HMG-I/HMG-Y Antibody (D-12) is a mouse monoclonal IgG2b κ HMG-I/HMG-Y antibody, cited in 1 publications, provided at 200 µg/ml
raised against amino acids 1-95 representing full length HMG Y of human origin
Anti-HMG-I/HMG-Y Antibody (D-12) is recommended for detection of HMG-l and HMG-Y of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
Anti-HMG-I/HMG-Y Antibody (D-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
TransCruz reagent for ChIP application (sc-393213 X, 200 µg/0.1 ml)
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HMG-I/HMG-Y (D-12): sc-393213.
m-IgG Fc BP-HRP and m-IgG2b BP-HRP are the preferred secondary detection reagents for HMG-I/HMG-Y Antibody (D-12) for WB and IHC(P) applications. These reagents are now offered in bundles with HMG-I/HMG-Y Antibody (D-12) (see ordering information below).
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
HMG-I/HMG-Y Antibody (D-12) is a high quality monoclonal HMG-I/HMG-Y antibody (also designated HMGA1 antibody, or HMGIY antibody) suitable for the detection of the HMG-I/HMG-Y protein of mouse, rat and human origin. HMG-I/HMG-Y Antibody (D-12) is available as both the non-conjugated anti-HMG-I/HMG-Y antibody form, as well as multiple conjugated forms of anti-HMG-I/HMG-Y antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. High mobility group (HMG) I (Y) chromatin proteins bind to the minor groove of AT-rich DNA sequences with high affinity. Evidence suggests that the binding of HMG proteins to DNA induces alterations in the DNA architecture including DNA bending and unwinding of the helix. HMG proteins synergize with Oct-2, members of the NFkB family, ATF-2 and c-Jun to activate transcription. Other studies indicate that phosphorylation of HMG protein is required to stimulate the transcriptional activity of the protein. Human HMG I (Y) contains two DNA-binding domains, termed HMG boxes. HMG proteins bind single-stranded DNA but induce conformational changes in double-stranded DNA alone.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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