Anti-HMG-I/HMG-Y Antibody (D-12) recommended for detection of HMG-l and HMG-Y of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-HMG-I/HMG-Y Antibody (D-12): sc-393213

Anti-HMG-I/HMG-Y Antibody (D-12) is rated 4.6 out of 5 by 8.
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Datasheets

    • Anti-HMG-I/HMG-Y Antibody (D-12) is a mouse monoclonal IgG2b κ HMG-I/HMG-Y antibody, cited in 1 publications, provided at 200 µg/ml
    • raised against amino acids 1-95 representing full length HMG Y of human origin
    • Anti-HMG-I/HMG-Y Antibody (D-12) is recommended for detection of HMG-l and HMG-Y of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-HMG-I/HMG-Y Antibody (D-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-393213 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HMG-I/HMG-Y (D-12): sc-393213.
    • m-IgG Fc BP-HRP and m-IgG2b BP-HRP are the preferred secondary detection reagents for HMG-I/HMG-Y Antibody (D-12) for WB and IHC(P) applications. These reagents are now offered in bundles with HMG-I/HMG-Y Antibody (D-12) (see ordering information below).
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Can this antibody be used to detect HMGA1?

Asked by: Chang Xing
Thank you for your question. HMG-I/HMG-Y Antibody (D-12): sc-393213 has been raised against full length HMG Y of human origin and recognizes HMG-I/HMG-Y, which is the protein that the HMGA1 gene encodes.
Answered by: Tech Support Europe
Date published: 2020-08-04

Can HMG-I/HMG-Y (D-12): sc-393213 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jerojero
Thank you for your question. Yes, HMG-I/HMG-Y (D-12): sc-393213 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 2 out of 5 by from no good signal in IF I didn't detect good staining signal in 4%PFA fixed embryonic mouse brain(after antigen retrieval)
Date published: 2018-03-07
Rated 5 out of 5 by from HMG-I/HMG-Y is good Works well for WB(in 1/1000), the antibody is high in potency and cear distinct bands, I think this antibody is very helpful to the experiment
Date published: 2017-10-18
Rated 5 out of 5 by from GOOD I used this antibody to detect MCF-7 WB (1:500), obtained clear bands
Date published: 2017-09-15
Rated 5 out of 5 by from Great antibody Great antibody binding HMG-I/HMG-Y for western blotting. used 1:800 dilution for mouse stomach tissue detection.The signal is very strong.
Date published: 2017-08-18
Rated 5 out of 5 by from antibody for wb I used this antibody to detect human breast cancer WB (diluted 1:200), obtained clear bands and strong signals.
Date published: 2016-12-17
Rated 5 out of 5 by from Functional antibody for IHC These days, I used this antibody for IHC (dilution 1:25) and got very special staining in human cancer. And I am going to use it as followed work!Thank you so much for your support!
Date published: 2016-11-10
Rated 5 out of 5 by from Exceptional immunoperoxidase nuclear and Exceptional immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human placenta tissue. -SCBT QC
Date published: 2015-05-07
Rated 5 out of 5 by from Clean bands in western blot analysis of HMG Clean bands in western blot analysis of HMG-I/HMG-Y expression in JAR and SK-OV-3 whole cell lysates. -SCBT QC
Date published: 2015-04-23
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