Anti-HMG-1/HMGB1 Antibody (J2E1) recommended for detection of HMG-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-HMG-1/HMGB1 Antibody (J2E1): sc-135809

Anti-HMG-1/HMGB1 Antibody (J2E1) is rated 4.5 out of 5 by 2.
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Datasheets

    • HMG-1/HMGB1 Antibody (J2E1) is a mouse monoclonal IgG2b, cited in 4 publications, provided at 50 µg/0.5 ml
    • raised against full length recombinant HMG-1 of human origin
    • recommended for detection of HMG-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HMG-1/HMGB1 (J2E1): sc-135809.
    • m-IgG2b BP-HRP is the preferred secondary detection reagent for HMG-1/HMGB1 Antibody (J2E1) for WB and IHC(P) applications. This reagent is now offered in a bundle with HMG-1/HMGB1 Antibody (J2E1) (see ordering information below).
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Dear SIr.. Can we use this HMG-1 Antibody (J2E1): sc-135809 antibody to detect bovine proteins???

Asked by: Ramya R
Thank you for your question. This antibody has not yet been tested in bovine samples so that is not a recommended species at this time. We are still gathering data to show on our website. Check back for updates!
Answered by: Tech Service
Date published: 2019-10-31

Can sc-135809: HMG-1 (J2E1) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenniferc
Thank you for your question. Yes, sc-135809: HMG-1 (J2E1) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Head Consultant It led a very good result in our research and we are in total very happy with good customer service Santa Cruz, it is the best company ever
Date published: 2017-01-02
Rated 4 out of 5 by from Clean bands in western blot analysis of HMG Clean bands in western blot analysis of HMG-1 expression in Jurkat and HeLa whole cell lysates. -SCBT QC
Date published: 2013-08-05
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