Anti-HMG-1/HMGB1 Antibody (HAP46.5) recommended for detection of HMG-1 of mouse, rat, human and Arabadopsis origin by WB, IP and ELISA

Anti-HMG-1/HMGB1 Antibody (HAP46.5): sc-56698

Anti-HMG-1/HMGB1 Antibody (HAP46.5) is rated 4.8 out of 5 by 17.
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Datasheets

    • Anti-HMG-1/HMGB1 Antibody (HAP46.5) is a mouse monoclonal IgG1 κ HMG-1/HMGB1 antibody, cited in 44 publications, provided at 100 µg/ml
    • raised against full length recombinant HMG-1 of human origin
    • recommended for detection of HMG-1 of mouse, rat, human and Arabadopsis origin by WB, IP and ELISA; also reactive with additional species, including canine
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HMG-1/HMGB1 (HAP46.5): sc-56698.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for HMG-1/HMGB1 Antibody (HAP46.5) for WB applications. These reagents are now offered in bundles with HMG-1/HMGB1 Antibody (HAP46.5) (see ordering information below).
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能否应用于coip有相关文献支持嘛?

Asked by: Zqq414666
感谢您的提问。HMG-1/HMGB1 (HAP46.5): sc-56698 还没有经过CoIP实验的验证,因此,目前不推荐应用于CoIP. Thank you for your inquiry. We have not yet tested HMG-1/HMGB1 (HAP46.5): sc-56698 on CoIP. As a result, CoIP is not currently a recommended application for this antibody.
Answered by: Sen Li
Date published: 2021-07-14

Does this antibody works well in Co-Immunoprecipitation experiments? What about immunocytochemistry?

Asked by: Dana I
Thank you for your question. We have not tested the HMG-1 Antibody (HAP46.5): sc-56698 for co-immunoprecipitation experiments, nor are we aware of anyone citing the antibody for this research application. The antibody is recommended and warrantied for immunoprecipitation of the HMG-1 antigen. We do not currently recommend this antibody for immunocytochemistry (or IF). However, there is a publication citing use of the antibody for this research application, see PMID: 24091659.
Answered by: Technical Support
Date published: 2017-02-06

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using withHMG-1 (HAP46.5): sc-56698?

Asked by: TinTin
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 13
Date published: 2017-01-13

Is this antibody reactive with avian samples?

Asked by: TinTin
Yes, this antibody is expected to pick up avian HMG-1. However, we recomment to contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 13
Date published: 2017-01-13
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Rated 5 out of 5 by from Very good This antibody is good in both sensitivity and specificity for detecting HMGB1.
Date published: 2021-08-19
Rated 5 out of 5 by from Great antibody Great antibody for WB. Used on PVDF membrane. Hela lysate and dilution 1/100 in blocking buffer 3%BSA 2% milk o/n 4C.
Date published: 2019-08-23
Rated 4 out of 5 by from Great My lab has purchased this antibody many times and we are very pleased
Date published: 2018-09-04
Rated 4 out of 5 by from Not very well. I couldn't get the predicted band at the dilution of 1:1000 in western blot,Maybe I should try again.
Date published: 2018-04-04
Rated 3 out of 5 by from works for WB I used this antibody for WB to test the expression of HMG-1 in 293T cells, the dilution ratio was 1:500. It works, but the bind was faint. I suggest reducing dilution ratio.
Date published: 2018-03-17
Rated 5 out of 5 by from good on WB I try this antibody on primary microglia and neuron to do Western Blot, the dilution is 1:400, It worked very good.
Date published: 2017-09-11
Rated 5 out of 5 by from Excellent Single band with out any background, excellent result
Date published: 2017-09-07
Rated 5 out of 5 by from good for wb it's great for western blot. the result can be used for paper
Date published: 2017-07-17
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