HLA-G Antibody (6D463)recommended for detection of denatured HLA-G heavy chain of human origin by WB, IP, IF and IHC(P)

HLA-G Antibody (6D463): sc-71263

HLA-G Antibody (6D463) is rated 4.0 out of 5 by 1.
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Datasheets

    • HLA-G Antibody (6D463) is a mouse monoclonal IgG1 provided at 100 µg/ml
    • raised against recombinant HLA-G denatured heavy chain denatured heavy chain of human origin
    • recommended for detection of denatured HLA-G heavy chain of human origin by WB, IP, IF and IHC(P)
    • See HLA-G (4H84): sc-21799 for HLA-G antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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Can sc-71263: HLA-G (6D463) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: TinTin
Thank you for your question. Yes, sc-71263: HLA-G (6D463) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Clean blot with single strong band at expected Clean blot with single strong band at expected size in human HLA-G transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-02-20
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