HLA-E Antibody (3H2679)recommended for detection of denatured heavy chain of HLA-E of human origin by WB, IP, IF and IHC(P)

HLA-E Antibody (3H2679): sc-71262

HLA-E Antibody (3H2679) is rated 3.2 out of 5 by 5.
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Datasheets

    • HLA-E Antibody (3H2679) is a mouse monoclonal IgG1 (kappa light chain) provided at 100 µg/ml
    • raised against recombinant human HLA-E denatured heavy chain denatured heavy chain
    • recommended for detection of denatured heavy chain of HLA-E of human origin by WB, IP, IF and IHC(P)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HLA-E (3H2679): sc-71262.
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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Can sc-71262: HLA-E (3H2679) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: TinTin
Thank you for your question. Yes, sc-71262: HLA-E (3H2679) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 1 out of 5 by from Tested on HFFF by FACS This antibody was tested on HFFF cells by FACS and did not give a signal, even at dilution 1:25
Date published: 2018-11-19
Rated 1 out of 5 by from Does not work for flow cytometry This HLA-E antibody does not work in my flow cytometry experiments
Date published: 2018-11-15
Rated 5 out of 5 by from Good staining No problem on human brain and tonsil IHC, demasking pH 9, 1:50 dilution.
Date published: 2017-11-08
Rated 4 out of 5 by from Performs well in Western Performs well in Western, good signal strength in in BJAB , K-562 , AML-193 , CCRF-CEM and MEG-01 whole cell lysates. -SCBT QC
Date published: 2015-03-23
Rated 5 out of 5 by from Clean blot with single strong band at expected Clean blot with single strong band at expected size using CCRF-CEM whole cell lysate. -SCBT QC
Date published: 2014-06-01
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