HLA-DR beta Antibody (HB10A)recommended for detection of HLA-DRβ of human origin by WB, IP, IF and IHC(P)

HLA-DRβ Antibody (HB10A): sc-73654

HLA-DRβ Antibody (HB10A) is rated 4.0 out of 5 by 3.
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Datasheets

    • HLA-DRβ Antibody (HB10A) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against HLA-DRβ of human origin
    • recommended for detection of HLA-DRβ of human origin by WB, IP, IF and IHC(P)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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Can HLA-DRβ (HB10A): sc-73654 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Randy McDonald
Thank you for your question. Yes, HLA-DRβ (HB10A): sc-73654 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from Strong band in WB in human HLA Strong band in WB in human HLA-DR 3 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-03-27
Rated 4 out of 5 by from Robust band in WB in BJAB whole cell lysate Robust band in WB in BJAB whole cell lysate. -SCBT QC
Date published: 2015-03-13
Rated 4 out of 5 by from Great immunoperoxidase cytoplasmic and membrane Great immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human tonsil tissue. -SCBT QC
Date published: 2015-02-24
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