HLA-DR Antibody (LN3)recommended for detection of HLA-DR of human origin by WB, IP and FCM

HLA-DR Antibody (LN3): sc-59252

HLA-DR Antibody (LN3) is rated 4.0 out of 5 by 2.
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Datasheets

    • HLA-DR Antibody (LN3) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against activated peripheral blood mononuclear cells of human origin
    • recommended for detection of HLA-DR of human origin by WB, IP and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See HLA-DR (520B): sc-69673 for HLA-DR antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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If I want to use HLA-DR (LN3): sc-59252 monoclonal antibody for Flow Cytometry, what starting dilution do you recommend I use?

Asked by: Dr Ninau Qelp
We recommend a starting dilution of 1µg of antibody per million cells. Here is a link to our recommended protocol: https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from Very large bands seen in Ramos Very large bands seen in Ramos, NAMALWA, BJAB, Daudi, HuT 78 and Raji whole cell lysates. -SCBT QC
Date published: 2015-05-14
Rated 4 out of 5 by from Robust indirect FCM data of human peripheral Robust indirect FCM data of human peripheral blood leukocytes stained with HLA-DR . -SCBT QC
Date published: 2014-12-10
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