HLA-DR Antibody (L243)recommended for detection of a nonpolymorphic HLA-DR epitope 1-3 of human origin by IF, IHC(P) and FCM

HLA-DR Antibody (L243): sc-18875

HLA-DR Antibody (L243) is rated 5.0 out of 5 by 7.
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Datasheets

    • HLA-DR Antibody (L243) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against human B lymphocytes
    • HLA-DR Antibody (L243) is recommended for detection of a nonpolymorphic HLA-DR epitope 1-3 of human origin by IF, IHC(P) and FCM; non cross-reactive with HLA-DQ or HLA-DP molecules
    • HLA-DR Antibody (L243) is available conjugated to agarose for IP; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to APC for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HLA-DR (L243): sc-18875.
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What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-01-20
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Rated 5 out of 5 by from Excellent! This antibody works great in western blot analysis of HLA-DR expression in BJAB, Raji, NAMALWA, Ramos and GA-10 whole cell lysates.
Date published: 2017-01-12
Rated 5 out of 5 by from Worked well in human samples for WB Worked well in human samples for WB.
Date published: 2015-10-06
Rated 5 out of 5 by from Superb immunofluorescence membrane staining Superb immunofluorescence membrane staining in methanol-fixed NAMALWA cells. -SCBT QC
Date published: 2015-05-19
Rated 5 out of 5 by from Good IF data with human monocytes Good IF data with human monocytes. -SCBT Publication Review
Date published: 2015-05-03
Rated 5 out of 5 by from Publishable WB data obtained using human Publishable WB data obtained using human leukemic T cells. -SCBT QC
Date published: 2015-02-01
Rated 5 out of 5 by from Good IHC data with paraffin Good IHC data with paraffin-embedded human renal tissues. -SCBT Publication Review
Date published: 2015-01-25
Rated 5 out of 5 by from Produced great FCM data for Ramos cells Produced great FCM data for Ramos cells. -SCBT QC
Date published: 2014-07-04
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