HLA-DR Antibody (Bra22)recommended for detection of HLA-DR and HLA-DP of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

HLA-DR Antibody (Bra22): sc-33718

HLA-DR Antibody (Bra22) is rated 5.0 out of 5 by 4.
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Datasheets

    • HLA-DR Antibody (Bra22) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against cells Reh-6 (non-T, non-B leukemia cell line)
    • HLA-DR Antibody (Bra22) is recommended for detection of HLA-DR and HLA-DP of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
    • HLA-DR Antibody (Bra22) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HLA-DR (Bra22): sc-33718.
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How can HLA-DR (Bra22): sc-33718 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Dr Ninau Qelp
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-33718 PE) or fluoroscein (sc-33718 FITC).
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Published data for WB in human DCs Published data for WB in human DCs. -SCBT Publication Review
Date published: 2015-05-12
Rated 5 out of 5 by from Strong signal in WB in human HLA Strong signal in WB in human HLA-DR transfected 293T and BJAB whole cell lysates. -SCBT QC
Date published: 2015-01-24
Rated 5 out of 5 by from Published data for WB in mouse DC2 Published data for WB in mouse DC2.4 dendritic cells. -SCBT Publication Review
Date published: 2015-01-15
Rated 5 out of 5 by from Produced superb FCM data for BJAB cells Produced superb FCM data for BJAB cells. -SCBT QC
Date published: 2014-06-11
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