HLA-DR Antibody (6A96)recommended for detection of HLA-DR of mouse, rat and human origin by WB, IP and FCM

HLA-DR Antibody (6A96): sc-71260

HLA-DR Antibody (6A96) is rated 4.0 out of 5 by 2.
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Datasheets

    • HLA-DR Antibody (6A96) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against activated peripheral blood mononuclear cells of human origin
    • recommended for detection of HLA-DR of mouse, rat and human origin by WB, IP and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See HLA-DR (520B): sc-69673 for HLA-DR antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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What is the recommended starting dilution for Western blot for HLA-DR (6A96): sc-71260 monoclonal antibody?

Asked by: Trav11
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-71260.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Positive WB data in human HLA Positive WB data in human HLA-DR transfected 293T cells and BJAB whole cell lysates. -SCBT QC
Date published: 2015-05-14
Rated 4 out of 5 by from Worked well for Western in Daudi Worked well for Western in Daudi, HuT 78 and Raji whole cell lysates. -SCBT QC
Date published: 2014-10-09
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