HLA-D/DR/DQ Antibody (35)recommended for detection of HLA-D, HLA-DR and HLA-DQ of human origin by IP, IF and FCM

HLA-D/DR/DQ Antibody (35): sc-53305

HLA-D/DR/DQ Antibody (35) is rated 2.0 out of 5 by 2.
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Datasheets

    • HLA-D/DR/DQ Antibody (35) is a mouse monoclonal IgG3 (kappa light chain) provided at 200 µg/ml
    • raised against tumor fragments of mouse origin
    • recommended for detection of HLA-D, HLA-DR and HLA-DQ of human origin by IP, IF and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HLA-D/DR/DQ (35): sc-53305.
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How can HLA-D/DR/DQ (35): sc-53305 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jerojero
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-53305 PE) or fluoroscein (sc-53305 FITC).
Answered by: Technical Support
Date published: 2017-02-28
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Rated 1 out of 5 by from Doesn't work with IHC We didn't receive a signal with IHC. We tried no demasking and demasking at pH 6 and 9. 1:50 dilution. 5 µm human tonsils and brain.
Date published: 2018-04-12
Rated 3 out of 5 by from Produced good indirect FCM data for human Produced good indirect FCM data for human peripheral blood leukocytes stained with HLA-D/DR/DQ . -SCBT QC
Date published: 2014-01-31
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