HLA-C Antibody (H-5)recommended for detection of HLA-C of human origin by WB, IP, IF, IHC(P) and ELISA

HLA-C Antibody (H-5): sc-166134

HLA-C Antibody (H-5) is rated 4.3 out of 5 by 6.
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Datasheets

    • HLA-C Antibody (H-5) is a mouse monoclonal IgG3 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 91-123 within an internal region of HLA-C of human origin
    • recommended for detection of HLA-C of human origin by WB, IP, IF, IHC(P) and ELISA
    • blocking peptide, sc-166134 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See MHC class I (W6/32): sc-32235 for HLA-C antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HLA-C (H-5): sc-166134.
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What application is the blocking peptide sc-166134 P appropriate for?

Asked by: AbPolly
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Tested on HFFF by FACS This antibody was tested on HFFF cells by FACS and did not give a signal, even at dilution 1:25
Date published: 2018-11-19
Rated 5 out of 5 by from Good staining No problem on human brain IHC, pH 9 demasking, 1:50 dilution.
Date published: 2017-11-08
Rated 5 out of 5 by from Great monoclonal for HLA-C Strong and specific, this is a very reliable antibody for both IHC and WB. Smart choice!
Date published: 2017-02-07
Rated 5 out of 5 by from Produced excellent immunoperoxidase cytoplasmic Produced excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human cervix tissue. -SCBT QC
Date published: 2015-05-02
Rated 5 out of 5 by from Strong band seen in WB in CCRF Strong band seen in WB in CCRF-HSB-2 whole cell lysate. -SCBT QC
Date published: 2015-04-06
Rated 5 out of 5 by from Good antibody for WB in non Good antibody for WB in non-transfected 293, human HLA-C transfected 293 and HeLa whole cell lysates. -SCBT QC
Date published: 2015-01-09
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